Journal: Frontiers in Cell and Developmental Biology
Article Title: Hoxa5 Activity Across the Lateral Somitic Frontier Regulates Development of the Mouse Sternum
doi: 10.3389/fcell.2022.806545
Figure Lengend Snippet: LPM-derived HOXA5 expression can be seen laterally as early as E11.5 and E12. (A – J ″ ) E12.5 Prx1-Cre-RFP embryo in which alternate sections were labelled for SOX9 (A – E ″ ) or HOXA5 (F – J ″ ) . Images are shown with RFP and either SOX9 (A – E) or HOXA5 (F – J) ; RFP alone (A ′– E ′; F ′– J ′ ) , or SOX9 (A ″– E ″ ) or HOXA5 (F ″– J ″ ) alone. Pink arrows in (A,B) indicate SOX9 expression associated with developing clavicles and IC elements. (K) Whole mount in situ hybridization for Hoxa5 in an E11.5 embryo. Black arrow indicates LPM expression anterior to the forelimb; red arrow points to LPM expression adjacent to the forelimb; green arrow marks additional HOXA5 expression that remains untranslated. (L,P) RFP and HOXA5 expression in sections through an E11.5 Prx1-Cre RFP embryo at the level indicated in (K) by black arrow (L) , or red arrow (P) . (M,N) expression of Hoxa5 (M) or EBF3 and HOXA5 (N) in the area indicated by blue square in (L) . (O) SOX9 expression in an alternate section of same embryo shown in (L) . (Q,R) expression of HOXA5 (Q) or EBF3 and HOXA5 (R) in area indicated by blue square in (P) . (S) SOX9 expression in an alternate section of same embryo shown in (P) . Yellow arrow in (N,R) indicates region of HOXA5 and EBF3 co-expression. Yellow arrow in (O,S) shows corresponding region of SOX9 expression. Inset in (O) shows SOX9 expression in the context of Prx1-Cre-RFP. Scale bar: (A – J) , 200 μM; (K) , 25 mm; (L – S) , 100 μM. SB, sternal bar; T1, thoracic rib 1.
Article Snippet: Primary antibodies: HoxA5 ( ; 1:5000); Sox 9 (Millipore-Sigma AB5535, 1:500 or RnD Systems AF3075-SP 1: 500); RFP (Chromotek 5F8, 1:1000); Ebf3 (RnD Systems AF5166, 1:1000); Tenascin (Sigma-Aldrich T3413, 1: 100); PCNA (Santa Cruz sc-56 1:200), Cleaved Caspase 3 (CST 9661 1:200).
Techniques: Derivative Assay, Expressing, In Situ Hybridization
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